aquilonis within the species complex) and Ceratitis capitata. tryoni(although indistinguishable from B. However, a slightly modified version ( Semeraro and Malipatil 2005) provided below, is recommended instead of Test 1 specifically for the diagnosis of the high-risk pest species B. Test 2 takes slightly longer, the RFLP patterns are sometimes more complex to interpret and the variation in size of the ITS1 region not as easy to detect is in Test 1. Therefore this test is recommended when the potential species is within that listed for this test. Test 1 is more rapid, the RFLP patterns less complex to interpret and the variation in size of the ITS1 region easier to detect than Test 2. Host records for the target taxa may assist in the elimination of possible non-target species.
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